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KMID : 1094720110160061248
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Biotechnology and Bioprocess Engineering
2011 Volume.16 No. 6 p.1248 ~ p.1253
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Immunostick assay for medical diagnosis of rheumatoid arthritis
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Ko Hyuk
Lee Eun-Hang
Jeon Byoung-Jin
Choi Hye-Jin
Kang Min-Jung
Pyun Jae-Chul
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Abstract
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An immunoassay based on stick-type solid support (immunostick assay) was developed. To demonstrate the medical diagnosis of rheumatoid arthritis (RA), cyclic-citrullinated peptide (CCP), one of specific antigens against RA autoantibodies, was immobilized on the surface of the immunostick, and a color index table was prepared for the determination of CCP-positivity of the test sera. The positivity of RA-positive (n = 31) and RA-negative (n = 20) sera was tested using the immunostick assay and a conventional enzyme-linked immunosorbent assay (ELISA). The statistical agreement of the test results from both methods was analyzed using inter-rater coefficient kappa and Bland-Altman test. The immunostick assay with a color index table was determined to have a high statistical correlation to the conventional ELISA method.
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KEYWORD
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immunoassay, immunostick, cyclic-citrullinated peptide, rheumatoid arthritis
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